Archived posting to the Leica Users Group, 2016/07/15
[Author Prev] [Author Next] [Thread Prev] [Thread Next] [Author Index] [Topic Index] [Home] [Search]Thanks Joe. You would not believe all the guesses as to what this was I have gotten. My sister-in-law said it looked like I took a picture from inside my garage looking at the door. Ha. So, you understand the thrill of weeks of putting stuff in and out of tubes, columns, devices and not knowing if you have anything in there or not until you finally are at a point to have enough to look at. Invisible stuff for the most part. Yes, indeed, the band was pretty. I have done a bit of protein work, but mostly DNA and RNA. Yes, EtBr is still used, though in the classroom, not so much. Syber Green or Gold is safer, and this photo is of a precast DNA gel from Lonza. I think they use EtBr but it is all self contained and proprietary info. Safe and quick but we cannot get at the band so will have to do agarose to cut it out to move on to our cloning step. Aram -----Original Message----- From: Joe Weil Sent: Thursday, July 14, 2016 4:23 PM To: Leica Users Group Subject: Re: [Leica] What does this say about me... It has been 20+ years since I did a gel for DNA. I am more familiar with SDS-PAGE for proteins. Do they still use ethidium bromide? That is a nice band on the right. I would cheer as well. Congrats. Joe -----Original Message----- >From: Aram Langhans <leica_r8 at hotmail.com> >Sent: Jul 14, 2016 2:20 PM >To: lug <lug at leica-users.org>, Leica Reflex <LeicaReflex at >freelists.org> >Subject: [Leica] What does this say about me... > >...when the last 4 weeks of work comes down to this photo to measure >success? > >http://gallery.leica-users.org/v/Aram/misc/m/cDNA+pcr+gut+male-9967.jpg.html > >It?s that white splotch on the right that we cheered at when we saw it. >Whew. Now we can move on to phase two of the project. > >Am I crazy? > >View large if you are a crazy as me. > >Aram > >Aram Langhans >(Semi) Retired Science Teacher >& Unemployed photographer > >?The Human Genome Project has proved Darwin more right than Darwin himself >would ever have dared dream.? James D. Watson > >_______________________________________________ >Leica Users Group. >See http://leica-users.org/mailman/listinfo/lug for more information