[Leica] Putting my microscope to work

Howard L Ritter Jr hlritter at bex.net
Sat Aug 13 14:22:57 PDT 2022


These are terrific, Aram! Take me back to my h.s. freshman biology days with Mr Wiley, Spirogyra, Euglena, and Paramecium.

Can you explain how you stack images (focused at different depths, I presume) to achieve greater depth of field without having everything muddled by the out-of-focus shallower and deeper layers of the subject?

—howard

> On Aug 13, 2022, at 3:24 PM, Aram Langhans via LUG <lug at leica-users.org> wrote:
> 
> I got an adapter so I can put my Nikon Z on my scope that I rebuilt a while back.  It works well.  I borrowed a few slides from the college and took some photos.  Due to the very shallow depth of field of a microscope, these are stacked images.
> 
> The hydrozoan Obelia is my favorite marine organism. Here is the feeding polyp at 100x.  8 images
> 
> Obelia Gastrozooid 100x-0971-Edit (leica-users.org) <http://gallery.leica-users.org/v/Aram/misc/sc/Obelia+Gastrozooid+100x-0971-Edit.jpg.html>
> 
> Here is the reproductive polyp also at 100x.  9 images
> 
> Obelia Gonozooid 100x-0979-Edit (leica-users.org)
> <http://gallery.leica-users.org/v/Aram/misc/sc/Obelia+Gonozooid+100x-0979-Edit.jpg.html>
> 
> And here is the small jellyfish that is the reproductive stage for Obelia.  9 images
> 
> Obelia Medusa 40x-0988-Edit (leica-users.org) <http://gallery.leica-users.org/v/Aram/misc/sc/Obelia+Medusa+40x-0988-Edit.jpg.html>
> 
> I am going to borrow a few more and have some fun before my summer class ends next week.
> 
> Comments welcome.
> 
> Aram
> 
> -- 
> Aram Langhans
> (Semi) Retired Science Teacher
> & Unemployed photographer
> “The Human Genome Project has proved Darwin more right than Darwin himself would ever have dared dream.”   James D. Watson
> 
> 
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